Describe, in detail, to a freshman undergraduate student the basic principle underlying the separation of dna using agarose gel electrophoresis. include the following in your description

Answers

Answer 1

Gel electrophoresis is a method for separating DNA, RNA, or protein fragments based on their molecular size from one another while being affected by an electric field.

What is Gel electrophoresis?In order to diagnose diseases, confirm the expression of proteins, or identify pathogens, electrophoresis is a laboratory procedure that separates molecules based on their size and electrical charge.The gel, which can be either polyacrylamide or agarose depending on the application, the electrophoresis buffer and vat, the molecular weight marker and fluorescent dye, as well as UV or LED light equipment, also known as a transilluminator, are needed for electrophoresis.

Learn more about electrophoresis here:

https://brainly.com/question/13949940

#SPJ4


Related Questions

According to piaget, organisms strive to achieve a balance of organized structures that provides effective ways of interacting with the environment. this is known as?

Answers

Piaget claimed that organisms strive to maintain the proper balance in their ordered structures so that they can interact with the environment effectively. What we refer to as equilibrium.

What is the Piaget theory attempting to explain?

The cognitive growth of children is described by Piaget's stage theory. Piaget believed that early cognitive development involved processes based on actions and later progressed to changes in mental operations. Cognitive development encompasses changes in cognitive process and capacities.

Piaget's concept of equilibrium is what?

All human mind, according to Piaget, strives for order and finds contradictions and inconsistencies in knowledge systems unsettling. In our cognitive systems, we therefore aim for "equilibrium." When a child's schemas are in a state of equilibrium, the majority of new information may be assimilated.

Learn more about Piaget's stage theory: https://brainly.com/question/10787379

#SPJ4

Consumer surplus is best described as the extra benefit consumers receive when they ________.

Answers

Consumer surplus is best described as the extra benefit consumers receive when they agreed to pay lower price than they would have been willing to pay.

The extra benefit that consumers gain when they agree to pay a lower price than they would have been willing to pay is best described as consumer surplus.

How significant is consumer surplus?Consumer surplus measures the benefit or gain that consumers derive from their purchases of goods and services. This additional benefit may take many different forms, such as lower purchase-related expenditures, more advantages from using or consuming something, or higher levels of experience satisfaction.Consumers who receive a significant advantage from their purchases are more likely to buy those same things again in the future, therefore consumer surplus is crucial for small firms to take into account.

To learn more about Consumer surplus, visit:

https://brainly.com/question/15415230

#SPJ4

When a child inherits one set of six hla genes together from one parent, this is called a(n):________

Answers

A child inherits one set of six hla genes together from one parent, this is called a(n):- haplotype.

How do HLA haplotypes get passed down?

The Mendelian inheritance of the HLA system results in considerable polymorphism, low recombination, no mutation has been seen in family studies, and antigen frequencies are known for a wide range of ethnic groups.

How many different HLA combinations exist?

Each parent passes on one haplotype to you. Consequently, there are four unique haplotype combinations from two parents. Inheritance of HLA follows a fundamental principle.

Do HLA proteins come from the parents?

Because HLA types are inherited, siblings may occasionally match. There is a 1 in 4 chance (or 25%) that each of your siblings and sisters who have the same parents as you is a complete or full match.

Describe HLA b20?

A human gene called HLA-B (major histocompatibility complex, class I, B) gives instructions for creating a protein that is essential for the immune system. A group of genes known as the human leukocyte antigen (HLA) complex includes the gene HLA-B.

To Know more about haplotype

https://brainly.com/question/15233229

#SPJ4

Select the correct order of changes that occur in the air tract when the diaphragm contracts :______.

Answers

The suitable order of adjustments that occur within the air tract when the diaphragm contracts: Contraction of external intercostal muscles > elevation of ribs & sternum > increased front-to-back dimension of thoracic cavity > the diaphragm relaxes, air passively leaves the lungs.

The diaphragm is a thin skeletal muscle that sits at the base of the chest and separates the abdomen from the chest. It contracts and flattens while you inhale. This creates a vacuum effect that attracts air into the lungs. when you exhale, the diaphragm relaxes and the air is driven out of the lungs.

The diaphragm is a barrier technique of beginning manipulation. it is reasonably effective, with a one-yr failure charge of around 12% with normal use. while you breathe in, your diaphragm contracts (tightens) and flattens, shifting down toward your stomach. This motion creates a vacuum on your chest, allowing your chest to enlarge (get bigger) and pull in air. when you breathe out, your diaphragm relaxes and curves again up as your lungs push the air out.

Learn more about diaphragm here:

https://brainly.com/question/4937745

#SPJ4

A woman is scheduled to undergo a modified radical mastectomy. which information would the nurse include when describing this surgery to the client?

Answers

A woman is scheduled to undergo a modified radical mastectomy. Removal of breast tissue, axillary nodes, and some chest muscles.

Mastectomy is the clinical time period for the surgical elimination of 1 or each breasts, partly or absolutely. A mastectomy is commonly accomplished to treat breast cancer. In a few instances, ladies believed to be at high hazard of breast most cancers have the operation as a safety measure.

Mastectomy is breast most cancers surgical procedure that eliminates the whole breast. A mastectomy might be completed: when a female can not be dealt with with breast-holding surgical procedure (lumpectomy), which saves most of the breast.

Mastectomies are very safe approaches, with minimum headaches. most of the people make a terrific recuperation and handiest need to stay in clinic for one night. however, a few human beings will want to spend some days in sanatorium. usually, it takes three to six weeks to completely get better.

Learn more about mastectomy here:-

#SPJ4

The legislation which banned discrimination in schools, employment, and public accommodations and secured the voting rights of african americans is the?

Answers

The correct answer is Civil Rights Act of 1964.

The Civil Rights Act of 1964, which prohibited discrimination based on race, color, religion, sex, or national origin and outlawed segregation in public places, is regarded as one of the civil rights movement's pinnacle legislative victories.

What is the Civil Rights Act of 1964?

Discrimination on the basis of race, color, religion, gender, or national origin is prohibited by the Civil Rights Act of 1964. The provisions of this civil rights legislation prohibited sex and race discrimination in hiring, promoting, and dismissing.

The act aimed to correct the legacy of discrimination against African Americans' voting rights. The measures were quickly challenged in court and sustained by the Supreme Court in a series of cases beginning in 1964.

To know more about the Civil Rights Act, visit:

https://brainly.com/question/1264052

#SPJ4

How does the police officers tone towards the woman change throughout the story ?

Answers

He talks down to the three women when he first engages them in conversation. His tone of voice and demeanor make this abundantly evident. The moment the women reveal to him that they are NASA employees and vital members of a research team, though, his perspective completely shifts. This can be interpreted in a variety of ways by the reader.

First off, the story takes place during a conversation time when integration was resisted in the Southern state where they lived. Additionally, there weren't many job opportunities for young women at the time. If they were educated, the majority of those who did not get married right away went on to become teachers, secretaries, or even nurses. Women who lacked the knowledge or skills required for those professions looked for domestic work.

But when they tell him they're from NASA, the dynamic between the officer and the women completely changes. First off, it makes the women conversation look better in his eyes. He understands that they are definitely well educated and in the scientific sector; they are neither lecturers or secretaries. Additionally, the space race was important to Americans at the time, therefore NASA held a special place in their hearts. So he wants to assist them in traveling to NASA so they may carry on with the crucial work they are doing for the nation.

Learn more about conversation here

https://brainly.com/question/3477680

#SPJ4

True or false? physical addresses must be converted to logical addresses in order to carry out the reference being made.

Answers

The statement indicating that 'physical addresses must be converted to logical addresses in order to carry out the reference being made.' is False.

What are computing physical addresses?

In computation, physical addresses refer to the address in the memory device in the computer (specific location in the CPU memory), whereas the logical address can be developed by the Central Processing Unit regarding a given computer program.

In conclusion, the statement indicating that 'physical addresses must be converted to logical addresses in order to carry out the reference being made.' is False.

Learn more about the CPU physical address here:

https://brainly.com/question/28126812

#SPJ1

In on the nature of things, the latin poet ______ argues that one need not fear death because all of reality, including the human soul, consists only of atoms.

Answers

In on the nature of things, the latin poet Lucretius argues that one need not fear death because all of reality, including the human soul, consists only of atoms.

Who is Lucretius?Titus Lucretius Carus was a poet and philosopher from ancient Rome. His only known work is the philosophical poem De rerum natura, which is typically translated into English as On the Nature of Things and somewhat less frequently as On the Nature of the Universe. It is a didactic work explaining the principles and philosophy of Epicureanism.The poem is the most comprehensive explanation of Epicurus' physical theory that has survived.One of the thinkers who declared war on religion due to its impact on society's citizens was Lucretius. He believed that religion just fools people into believing in things that do not actually exist and does not have any good effects on a person's life.

Learn more about Lucretius here:

https://brainly.com/question/10231231

#SPJ4

What is the word used to describe something valuable that each party exchanges in order to demonstrate that they agree to the contract's terms?

Answers

The consideration is the  word used to describe something valuable that each party exchanges in order to demonstrate that they agree to the contract's terms.

What is meant by contract?


This is the term that is used to refer to the legal agreement that exists between two parties that would have them agreeing to the terms and the conditions of a business. The contract helps to take care of the business arrangement between the two parties involved and it is enforceable.

Hence we can say that The consideration is the  word used to describe something valuable that each party exchanges in order to demonstrate that they agree to the contract's terms.

Read more on contract here: https://brainly.com/question/5746834

#SPJ1

The year 2013 contains four digits whose values are consecutive integers (0, 1, 2, 3). how many years after 2013 will this event occur next?

Answers

The The year 2013 contains four digits whose values are consecutive integers (0, 1, 2, 3). The number of years after 2013 that this event occur next is 18 years.

What are consecutive integers?

It should be noted that consecutive integers simply has to do with the numbers that follow each other. An example of consecutive numbers are 234 or 798. They can be arranged in such a way that they follow each other.

It should be noted that 2013 has consecutive numbers. In this case, the numbers are 0, 1, 2, 3. Therefore the year when this will occur again will be in 2031. This was gotten by rearranging the numbers from 2013.

Then we will find the difference between tye numbers. Therefore, this will be:

= 2031 - 2013

= 18 years.

Therefore the number of years is 18 years.

Learn more about integers on:

brainly.com/question/24552134

#SPJ1

What piece of equipment is incuded in every level of prehospital emergency trasining?

Answers

What piece of equipment is included in every level of prehospital emergency training Automated external defibrillator.

The requirements for prehospital emergency care and the individuals who offer it are governed with the aid of the legal guidelines in every nation and are typically regulated with the aid of a state workplace of EMS.

Prehospital care manner the emergency clinical services rendered to an emergency-affected person for analytic, resuscitative, stabilizing, or preventative purposes, precedent to and for the duration of transportation of the such patient to fitness care centers.

Prehospital CPR duration become defined as the time from CPR initiation by means of EMS employees to the time of the prehospital return of spontaneous flow or to the time of clinic arrival.

Learn more about prehospital emergencies here:-https://brainly.com/question/28430282

#SPJ4

Psychologists use different terms such as frequency and pitch and wavelength and hue because:_________

Answers

Psychologists use different terms such as frequency and pitch and wavelength and hue because the two worlds physical and psychological were not identical.

Who are Psychologists?A psychologist is a specialist in the field of psychology who focuses on understanding and analyzing people's thoughts, feelings, perceptions, actions, and social interactions. The testing, observation, and interpretation of how people interact with one another and their circumstances are frequent parts of their work.Psychologists can provide tests and examinations that can aid in the diagnosis of conditions or reveal more about a person's thoughts, feelings, and behaviors. They can also assist patients learn to manage their chronic illnesses, overcome addictions, and cope with stressful situations. For help with mood-related issues including sadness, mania, anger, or , people often consult a clinical psychologist. Others might need help coping with difficult times or events in life (e.g., marriage, divorce, new employment, grief). Unresolved traumas for some people require support.

Learn more about Psychologists here:

https://brainly.com/question/3833605

#SPJ4

Historically, which stage was first in the development of a nursing theory and philosophy?

Answers

Theorizing was the first stage in the development of a nursing theory and philosophy.

Theorizing, creating a syntax, creating concepts, and philosophical discussion were all milestones in the creation of the nursing discipline.

These phases contributed to the development of the discipline's traits as a human science, a practise science, a science with social objectives to enable nurses to deliver effective and high-quality care, and a science where gender differences and the need for understanding minorities are key areas of focus.

In order to fulfil the integrative and multidisciplinary nature of these demands, future theoretical growth in the subject will be motivated by population needs. Practice, theory, and research are the sources of future theory.

"A creative and rigorous structure of ideas that project a tentative, deliberate, and systematic understanding of reality" is how nursing theory is described. Nurses are able to acquire information relevant to enhancing patient care through methodical inquiry, whether in nursing research or practise.

To learn more about nursing theory, refer

https://brainly.com/question/28346290

#SPJ4

The term ________ refers to the process of converting the physical energy produced by a stimulus into electrochemical messages.

Answers

The term sensation refers to the process of converting the physical energy produced by a stimulus into electrochemical messages.

The field of physical chemistry known as electrochemistry studies how a recognisable chemical change and an electrical potential difference interact as a measurable and quantitative phenomenon, with the potential difference either resulting from or being a cause of the chemical change. These reactions include the movement of electrons between electrodes separated by an ionically conducting and electronically insulating electrolyte, generally an external electrical circuit but not always, as in electroless plating (or ionic species in a solution).

Learn more about Electrochemical here

brainly.com/question/12034258

#SPJ4

Compare the initial colonization of brazil and mexico and the larger imperial projects of portuigal and spain.

Answers

Differences between the Portuguese colonisation of Brazil and larger imperial projects of spain were significant.

Spanish empire was a colonial empire ruled between 1492 and 1976 by Spain and its antecedent kingdoms. One of the biggest empires in history, it co-founded the European Age of Discovery with the Portuguese , With the so-called Iberian union project , which many historians view as a Spanish conquest, Philip II of Spain took control of Portugal and its overseas territories in 1581 following the Spanish victory in the War of the Portuguese Succession.

The Portuguese saw Brazil as a trading port rather than a destination for huge numbers of settlers because they were more interested in evangelization and trade in Asia and Africa, which included trafficking in enslaved people. The Portuguese engaged in trade with native Brazilians and smaller-scale fights against competing groups instead of mounting massive military expeditions to conquer entire empires.

To learn more about lberion union project, refer this link.

https://brainly.com/question/27366437

#SPJ4

Free and fair elections are conducted in India. Do you agree? Justify.

Answers

Yes because they’re all equal

A(n)____is a grouping of tissues that forms a distinct structure (for example, a heart), which performs a specialized task. listen to the complete question

Answers

A(n) organ is a grouping of tissues that forms a distinct structure (for example, a heart), which performs a specialized task.

What is organ?

Organ of the body play a major role in the body as this organ are tissues that enables us to function properly and live.

This organ perform important function or specialized function in the body.

Some examples of the organs of the body are:

BrainLungsHeartEyeKidneys Liver  etc

The above listed examples of organ are crucial as they each has the function they perform in the body.

Therefore A(n) organ is a grouping of tissues that forms a distinct structure (for example, a heart), which performs a specialized task.

Learn more about organ here:https://brainly.com/question/545314

#SPJ1

What segment of u.s. society represents a challenge to the social security system?

Answers

The social security system is put under pressure by the baby boomer generation sector of American society.

What do you understand by  social security system?

In the United States, Social Security is a federal program that offers retirement benefits and disability income to eligible individuals, as well as to their spouses, children, and survivors. To be eligible for retirement benefits, employees must be at least 62 years old and have paid into Social Security for at least ten years.

Types

Retirement benefits under social security.

Disability benefits under social security.

Social Security benefits for dependents.

Benefits for Social Security Survivors.

Social Security Benefits for Spouses of the Same Sex.

To learn more about social security system, Visit: https://brainly.com/question/14420288

#SPJ4

The ______________ is a governmental policy based on the belief that controlling drug availability will solve drug problems.

Answers

The war on drug  is  based on the belief that controlling drug availability will solve drug problems.

What is governmental policy?

Government policies  can be described as the policies that gives the  reasons things are to be done in a certain way , it helps to express the  a course of action  as well as to  create a starting point for change

It should be noted that The war on drug  is  based on the belief that controlling drug availability will solve drug problems.

Find out more on the Government policies at https://brainly.com/question/18464634

#SPJ1

The conclusion drawn from meta-analyses and large-scale studies that all major psychotherapies yield comparable effect sizes is referred to as the?

Answers

The conclusion drawn from meta-analyses and large-scale studies that all major psychotherapies yield comparable effect sizes is referred to as the client variables.

What is known as psychotherapies?

This refer to the process of treating psychological disorders and mental distress through the use of verbal and psychological techniques. During the psychotherapies process, a trained psychotherapist will helps the client tackle specific or general problems such as a particular mental illness or a source of life stress.

But depending on the approach used, the wide range of techniques and strategies can be used and almost all types of psychotherapy involve developing a therapeutic relationship, communicating and creating a dialogue, and working to overcome problematic thoughts or behaviors.

Read more about psychotherapies

brainly.com/question/4424685

#SPJ1

What have some states done to address rapidly rising premiums for medical malpractice insurance?

Answers

The Type of Policy , Location of the Practice are some of the address that rapidly rising premiums for medical malpractice insurance.

What does malpractice insurance serve?

Medical malpractice insurance, a specialized form of professional liability insurance, protects doctors and other healthcare providers from responsibility resulting from disputed services that cause a patient's harm or death.

What constitutes malpractice in its three elements?

(1) A professional obligation due to the patient

(2) A breach of that duty

(3) Damages stemming from the breach

To know more about insurance visit :

https://brainly.com/question/27822778

#SPJ4

If you have a metamorphic rock, and you bury it and heat it until it melts, and then bring it closer to the surface where it cools and solidifies again, the resulting rock would be _______.

Answers

igneous rock
this is because igneous rock is solidified magma

____ became the first noted european business traveler as he pioneered trade routes from europe to china between 1275 and 1292.

Answers

Marco Polo became the first noted European business traveler as he pioneered trade routes from Europe to china between 1275 and 1292.

Marco Polo changed into a Venetian merchant, explorer and author who travelled via Asia alongside the Silk road among 1271 and 1295.

Why is Marco Polo so famous?

Marco Polo turned into recognized for the e-book, "The Travels of Marco Polo", which describes his voyage to and studies in Asia. Marco Polo traveled notably with his circle of relatives, journeying from Europe to Asia from 1271 to 1295 and final in China for 17 of these years.

What did Marco Polo accomplish?

Marco Polo is known for travelling along the Silk avenue to China, in which he explored and documented an awful lot of Asia now not yet explored with the aid of Europeans.

What clearly took place to Marco Polo?

Marco Polo died at his domestic in Venice on January 8, 1324. As he lay dying, friends and lovers of his e-book paid him visits, urging him to confess that his book turned into fiction. Marco Polo would not relent.

Learn more about Marco Polo here:- https://brainly.com/question/1961580

#SPJ4

What reasons for writing the constitution are given in the preamble to the constitution? choose three options.

Answers

Although the options are not specified in the question, here are all the reasons for writing the Constitution of the US given in the preamble:

Form a more perfect Union of states Have internal peace and tranquility Organize and create a common defense of the statesseek general Welfare and prosperity.guarantee "the Blessings of Liberty" for future generations

The preamble is a short paragraph at the beginning of the Constitution that introduces the most important law of the USA. It does not state laws, duties, or guarantees. It expresses the aim of the Constitution, the values, and the spirit of the founding fathers with which the text should be interpreted.

In spite of not having the list of options, any of the ones listed above would be correct.

You can learn more about the US Constitution in the link below:

https://brainly.com/question/28454530

#SPJ4

De groot (1966) examined memory for logical and illogical chess positions. the results were that?

Answers

De Groot (1966) examined memory for logical and illogical chess positions. the results were that 28341109.

Chess gamers have an advantage in remembering the positions of portions at the board because they could set portions into significant corporations. Chess masters can keep in mind extra pieces compared to beginners from a chess board that could arise in a real chess sport however now not from a board with randomly-located chess quantities.

Reminiscence is extremely essential, inside the calculation need to keep all analyses and sub-versions and determine which one is the maximum correct and achieved the move; sample popularity can be very crucial and the greater styles you understand the higher the Chess participant, the whole thing in chess calls for reminiscence.

Learn more about chess here:-https://brainly.com/question/13701946

#SPJ4

A social consequence of introducing coffee into the highlands of papua new guinea was that?

Answers

A social consequence of introducing coffee into the highlands of Papua new guinea was that people had greater access to commodities, young men gained social status, and coffee plantations engulfed all available land.

The highlands run east-west across the island, which is politically divided between Indonesia in the west and Papua New Guinea in the east.

Positive social effects are often subtle and unnoticed (inclusion in a group, social connectedness, or an increase in peer approval), but they encourage future use. Negative social consequences (peer disapproval of use, odor, lack of motivation) may be ignored or attributed to other causes.

To know more about Papua New Guinea, click here.

https://brainly.com/question/12665689

#SPJ4

The oceania theory is based on the notion that cultural and linguistic similarities exist between native south american peoples and those from:________

Answers

The Oceania theory is based on the notion that cultural and linguistic similarities exist between native south American peoples and those from Australia and Polynesia

This is further explained below.

What is Oceania's theory?

Generally, The Oceania idea proposes that our ancestors traveled over the Pacific Ocean, landed in South America, and then migrated their way up the continent.

DNA study reveals that there may have been a civilization that existed before the Clovis culture.

Monte Verde is at least a thousand years older than any Clovis site, making it the oldest of the bunch.

In conclusion, The Oceania hypothesis postulates that aboriginal populations of South America share linguistic and cultural traits with those of Australia and Polynesia.

Read more about the Oceania theory

https://brainly.com/question/11934538

#SPJ1

Why is there such a measurable difference between the first and second weighing of the crucible for run 3?

Answers

This is because the crucible would weigh more and, when it was eventually burned off along with the remainder of the water, would result in a greater perceived loss. Before its mass is measured, the burned crucible is handled with greasy fingertips.What is crucible?A crucible is a ceramic or metal vessel used to melt metals or treat other substances to extremely high temperatures. Crucibles can be produced from any material that can endure temperatures high enough to melt or otherwise change their contents, despite traditionally being most frequently built of clay. As a vessel for melting or testing metals since antiquity.

Learn more about crucible here:

https://brainly.com/question/10237849

#SPJ4

What environmental force involves developing applications from science or engineering research?

Answers

A technology is the environmental force that involves developing applications from science or engineering research.

What is a technology?

This refers to the process of using human knowledge to turn resources into goods and services that people need. Invention. All these new device, method or process that has been developed are as a result of advancement in technology.

The significance of technology in the market can not be underemphasized, it is imperative to fully understand the meaning of environment forces. In this sense, the environment involves those the environmental forces or factors that indirectly alter the operations of an organization.

In this modern world, the leading forces in the environment sense is technology, this is because of its influence on the buyer’s choices. In essence, a buyer today has a range of choices unlike before the development of technology. The internet is the world hub of information that can either benefit or disadvantage an organization.

Read more about technology

brainly.com/question/25110079

#SPJ1

Other Questions
Exercise 2 Write a complete thesis statement from the theme given below.theme: the importance of rain forests for new medicines Select the correct answer.It is okay to use a pool noodle when learning to tread water.A. TrueB. False The fact that 45-year-old george is worried about how the election of the new u.s. president will impact his current job status is best associated with? Why do many people find it more challenging to learn to sew nowadays than in generations past?Sewing involves much less technology than it used to.People today buy fewer items of clothing than most people used to.Unlike in past generations, most people today have no idea how to sew.Computerization of sewing machines has become less popular. do multiple sclerosis symptoms moderate the relationship between self-efficacy and physical activity in people with multiple sclerosis? Fill in the blank if sin=3/5, then cos= Interest payable, salaries payable, and accounts payable are common examples of items classified as:______ Arun says if he adds together two mixed numbers, his answer will always be less than the sum of the whole-number parts plus 1. Say 2 counter-examples to show Arun's statement is not true. A counter statement is any example that shows a statement is false If M (-2, 5) is the midpoint ofCD and the coordinates of C are (4, 7), what are the coordinates of D? Please help this is an Earth Science question An individual breaks a large cash deposit into several smaller deposits with the intention of evading the filing of a currency transaction report. this is called:________ An increase in business taxes causes a(n) ______ in supply and will ______ production costs. What is Okonkwo referring to when he is concerned about his ch?O His red hut earth.O His personal spiritual being.O His snuff folder.O His ceremony to give thanks. What would be the efect on the properties of the water molecule if oxygen and hydrogen? Prime Factorization36=48= Evaluate the function.f(x) = -x + 7xFind f(-1) use benchmarks to estimate the sum or difference-1) 17/18-3/7 The traditional channel of initial communication between the patient and the physician is:_____. The nurse is inserting a foley catheter for a client. which nursing action is appropriate if the sterile field is broken during this procedure? Solve for the value of q. 780 (59+2)