A protein with a high percentage of aspartate and glutamate residues would be best purified and concentrated with which type of column?.

Answers

Answer 1

Anion-exchange chromatography would best purify and concentrate a protein with a high percentage of aspartate and glutamate residues.

Anion-exchange chromatography separates chemicals based on their charges by utilizing an ion-exchange resin with positively charged groups, such as diethyl-aminoethyl groups (DEAE). The resin is covered with positively charged counter-ions in solution (cations). Anion exchange resins attach to charged molecules and dispense the counter-ion negatively. At higher pH levels, anion exchange chromatography is often used to filter proteins, amino acids, sugars/carbohydrates, and other acidic compounds having a negative charge. The intensity of the substance's negative charge determines the binding's tightness between the substance and the resin.

Thus, aspartate and glutamate, negatively charged amino acids would bind to the positively charged resin in the column.

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Related Questions

In times of carbohydrate deprivation, the body can create glucose from amino acids in a process called:________

Answers

I think it’s gluconeogenesis

Sexually reproducing organisms produce ____________ gametes when ____________ cells undergo meiosis.

Answers

Answer:

haploid gametes and germ cells

The parasite ____ can be spread in water supplies, water supplies, water parks, and community swimming pools.

Answers

Water supplies, water parks, municipal swimming pools, and water supplies are all potential breeding grounds for Cryptosporidium.

What is parasite?

A parasite is an organism that coexists with another creature in order to gain nutrition, grow, or reproduce—often in a way that is harmful to the host directly or indirectly.

Water supplies, water parks, municipal swimming pools, and water supplies are all potential breeding grounds for Cryptosporidium. It causes a diarrheal disease.

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Because muscle contractions often need to happen quickly, they are controlled via _____________ receptors.

Answers

Because muscle contractions often need to happen quickly, they are controlled via ionotropic receptors.

What is ionotropic?

A class of transmembrane ion channels known as ionotropic receptors respond to the binding of a chemical messenger (ligand), such as a neurotransmitter, by opening or closing the channels. Endogenous ligands often attach to LGIC protein complexes at an allosteric binding site, which is situated on a separate part of the protein than the ion conduction pore. The ion channel's activity is controlled by a ligand, and it typically exhibits high selectivity for one or more ions, such as Na+, K+, Ca2+, or Cl-.

Nicotinic acetylcholine receptors are the standard example of ligand-gated ion channels. It is made up of five protein subunits called pentamers, each of which has two cholinergic binding sites that, when occupied, change the receptor's structure and allow an internal pore to open.

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If a dna-binding protein "reads" a short stretch of dna and detects the following "second" genetic code provided by the functional groups located on each base as h-hd-ch3-ha-ha-ha-ha-hd, then what is the corresponding sequence of bases?.

Answers

If a DNA-binding protein "reads" a short stretch of DNA and detects the following "second" genetic code provided by the functional groups located on each base as H-HD-CH3-HA-HA-HA-HA-HD, then C-T-G-A is the corresponding sequence of bases.

Proteins that bind to DNA and RNA make up a sizeable portion of biological proteins and play crucial roles in cells. Controlling transcription and translation, DNA repair, splicing, apoptosis, and modulating stress responses are just a few of their duties.The functions of DNA are modulated by the hundreds of proteins that are present in our cells. Due to their ability to bind to DNA, these proteins are known as DNA-binding proteins. DNA-binding proteins perform a variety of tasks, including as regulating the synthesis of proteins, controlling cell growth and division, and storing DNA inside the nucleus.Adenine (A), cytosine (C), guanine (G), and thymine (T) are the four bases of DNA (T). These bases come in particular pairs (A with T, and G with C).

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A blood alcohol content (bac) of ______ or higher produced driving impairment in all people.

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A blood alcohol content (bac) of 0.5 or higher produced driving impairment in all people.

Blood Alcohol Content- The amount of alcohol in the blood or on the breath is known as the blood/breath alcohol concentration (BAC). The mass of ethanol, in grams, in 100 milliliters of blood or 210 liters of breath is used to measure blood alcohol content (BAC). Blood, urine, or breath tests can be used to determine BAC.

Blood alcohol levels depend on a variety of variables, including the quantity of drinks consumed, body weight, gender (females have greater blood alcohol levels than men when they consume the same amount of alcohol), and number of drinks.

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A drop of blood is added into a solution and found that red blood cells will hemolyse. what solution it might be?
apply the concept based on the results observed in the lab experiment.

Answers

The solution might be hypotonic.

When a drop of blood is added in the hypotonic solution, it may hemolyse Or bursting of cell take place, this is because the solvent comes inside the cell due to lower concentration of solute and it becomes hemolysed.

There are three types of solutions.

Hypertonic condition of the solution occurs when the concentration of solute outside is higher than that inside the cell. Hypotonic condition of the solution occurs, when the concentration of solute outside is lower than that inside the cell. Isotonic condition of solution occurs when concentration of dissolved solute outside is equal to the concentration of solute inside the cell.

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A temperate biome with high temperatures and little precipitation is a

tropical grassland
tundra
coniferous forest
chaparral

Answers

A tropical grassland is a temperate biome with high temperatures and little precipitation (option A).

What defines a tropical grassland?

A grassland is defined as an area where grasses predominate over large shrubs or trees. A grassland can either be tropical or temperate depending on the temperature.

The tropical grassland also called savanna is a grassland with few scattered trees. This is due to the relatively low amount of rainfall/precipitation per annum. The tropical grassland is also characterized by high temperatures and low rainfall.

Therefore, it can be concluded that a temperate biome with high temperatures and little precipitation is a tropical grassland.

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Most viruses form a capsid around their nucleic acid core. this capsid is composed of?

Answers

I think it’s Protein

Metabolic rate of alcohol is unaffected by exercise. group of answer choices true false

Answers

Metabolic rate of alcohol is unaffected by exercise TRUE.

The basal metabolic rate (BMR) calculates the bare minimum of calories required for a person to maintain their essential bodily functions over the course of a 24-hour period of rest. Such processes include breathing as an example. circulation. digestion as well as nutrient absorption

The three elements that make up the metabolic rate are the baseline metabolic rate, the energy expended during physical activity, as well as the thermic effect with food.

Alcohol reduced shivering metabolic rate by an overall mean of 13%, insufficient to affect cooling rate.

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If nucleotides from the dna of a human were quantified and 30 percent of them contained the base adenine, what percentage of them would contain the base guanine?.

Answers

If 30% of the DNA is Adenine, then 20% will be guanine.

What is chargaff's rule?

The molar ratios [A] = [T] and [C] = [G] were present in all species, and the ratio [C + G] / [A + T] was often lower than unity since [C + G] is a less common element. And Chargaff's rule name is given to these ratios.

Additionally, the molar similarities of A with T and C with G naturally support a certain type of combination link. Furthermore, Chargaff himself is unwilling to take a chance on claims of his experimental observation in the absence of new experimental ideas.

Additionally, he believed that Watson and Crick's model-building strategy was not grounded in science. Most notably, he later disparaged molecular biology in general and eventually grew frustrated at what he perceived as a refusal to acknowledge the significance of his work.

Therefore, in this case, 30% is Adenine and 30% will be Thymine, so the remaining 40% will be GC, out of which 20% will be Guanine.

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Which two statements correctly describe the relationship between science and technology

Answers

Answer:

The answer is C and D

Explanation:

The two statements that correctly describe the relationship between science and technology are

C. Scientific discoveries can be made as a result of new technology.

D. Scientific discoveries influence the development of new technology.

What are science and technology?

Technology and science mutually advance one another. The development of new technologies is based on scientific understanding. Scientists are frequently able to examine nature in novel ways and create novel findings thanks to new tools.

The telescope and microscope are two examples of technology that have advanced science. Technology is the use of knowledge to accomplish practical objectives in a dependable and repeatable way. The result of such an effort is also referred to as technology.

Science, industry, communication, transportation, and daily living all make extensive use of technology.

Therefore, the correct options are C and D.

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For a patient with respiratory acidosis chronic compensation by the body will include?

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For a patient with respiratory acidosis chronic compensation by the body will include protein buffering.

What is protein?

Protein is defined as the large macromolecules and biomolecules that are made up of long chains of amino acid. There is various function of protein such as DNA replication, speeding up chemical reaction, and helps to maintain the structure of cell.

The main function of protein is to maintain the structure and function of organs, repair muscle, and muscles. Diet contain high protein is recommended for the purpose of losing weight, reducing fat, or increasing fat.

There are several types of protein such as structural protein, transport proteins, antibodies, contractile proteins, hormonal proteins, and enzymes.

Therefore, For a patient with respiratory acidosis chronic compensation by the body will include protein buffering.

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When environmental conditions around a bacterium are either a neutral ph or an alkaline ph, the surface of a bacterium will:__________-

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When environmental conditions around a bacterium are either a neutral ph or an alkaline ph, the surface of a bacterium will: have a negative charge

To identify if a bacterium has a negative charge a gram stain must be performed, this is a rapid laboratory test used to detect the presence of bacteria that cause an infection, if the result of the gram stain is red the bacteria are gram negative.

What is a bacteria?

It is a microorganism formed by a single cell, of small size. Its cellular structure is prokaryotic and it is characterized by not having a nucleus.

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Which of these traits have been found by twin studies to be strongly hereditary? liking of spicy foods dislike of romance movies dancing ability schizophrenia stubbornness

Answers

Schizophrenia traits have been found by twon studies to be strongly hereditary.

What is hereditary?

Hereditary theory holds that many physical characteristics can be passed down from generation to generation via DNA, by passing on the set of instructions or genes in your DNA to your offspring.

Heredity in biology refers to the transmission of genetic factors from parents to offspring or from one generation to the next. The biological definition of heredity may also refer to the association of a specific trait with an individual organism's genetic constitution rather than environmental conditions.

Eye color is an example of DNA.

There are four types of hereditary

Dominant, recessive, Co dominant, intermediate.

It is more likely that different combinations of genes make people more vulnerable to the condition. However, having these genes does not guarantee that you will develop schizophrenia. Twin studies provide evidence that the disorder is partially inherited. The same gene is shared by identical twins.

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that occur(s) when atoms share
electrons unequally is/are called
generally produce(s)
that occur(s) as weak attractions
between dipoles is/are called
that occur(s) when atoms share
electrons equally is/are called
generally produce(s)
that occur(s) when atoms
transfer electrons is/are called
generally produce(s)

Answers

A polar bond  that occur(s) when atoms share electrons unequally.

A Nonpolar Covalent Bond occur(s) when atoms share electrons equally.

What is a Nonpolar Covalent Bond?

This is known to be a bond that is said to have  created if atoms are said to share their electrons in an equal way.

Note that this is one that often  occurs if two atoms are said to be the same or are similar or have the similar electron affinity.

Note also that the closer the values of a given electron affinity, the stronger the attraction.

Therefore, A polar bond  that occur(s) when atoms share electrons unequally.

A Nonpolar Covalent Bond occur(s) when atoms share electrons equ

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The bacterial flagellum is driven to rotate by protons moving down a charge and ph gradient and the energy provided is called the?

Answers

The energy generated by protons travelling down a charge and ph gradient to rotate the bacterial flagellum is known as the proton motive force.

A proton motive force

In the mitochondria of most tissues, the proton-motive force produced by the respiratory chain complexes is mostly employed to move protons through the ATP synthase complex, where they are converted into ATP from adenosine diphosphate (ADP) and phosphate.

Is there a pH gradient in proton motive force?The proton motive force is produced by an electrochemical gradient that has both chemical and electrical components. The pH gradient, or varying proton concentrations, is what causes protons to migrate across the membrane with a movement of molecules from one side to the other and is what makes up the chemical component.

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What are ocean animals' biological clocks in tune with so that their lives are not upset by high and low tides?


A. Lunar rhythm

B. Annual rhythm

C. Seasons

D. Rain schedule

Answers

The ocean animals also keep time using the  Lunar rhythm.

What are ocean animals?

The ocean animals are the animals that can be found in the ocean. We know that the ocean is salt water. Another name for ocean animal are marine animals. In the Marine ecosystem there are high and low tides.

More recent scientific evidence has shown that, aside from the internal biological clocks of the organisms and that of the rising and falling tides. The ocean animals also keep time using the  Lunar rhythm.

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Answer:

A. Lunar rhythm

Explanation:

Scientific evidence has shown that, aside from the internal biological clocks of the organisms and that of the rising and falling tides, the ocean animals also keep time using the Lunar rhythm.

The brain's right hemisphere is best described as _____ the left hemisphere's processing.

Answers

The brain's right hemisphere is best described as complementing the left hemisphere's processing.

What does the right hemisphere of the brain control?

The right side of the brain significantly controls the left side of the body and the left side of the brain generally regulates the right side of the body. Due to this, the damage to one side of the brain is going to influence the opposite side of your body.

The two hemispheres of the brain are known as the left hemisphere and the right hemisphere. Both of them are connected with the help of a thick band of nerve fibers called the corpus callosum.

Both regions of the brain are capable to communicate with each other through this bridge. It also regulates the complementing process between the two hemispheres of the brain.

Therefore, the brain's right hemisphere is best described as complementing the left hemisphere's processing.

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The non-mendelian inheritance pattern observed in snail coiling is most related to ______.

Answers

The  non Mendelian inheritance pattern observed in snail coiling is most related to maternal inheritance.

Why is genetic inheritance important?

Genetic inheritance is necessary for evolutionary change. It describes how genes are passed from one generation to the next.It might consequently initially seem surprising then, that genetic inheritance itself is rarely inculded in life research.  

The phenotype controlled by organelle genes exhibit maternal inheritance.The classic phenotype which exhibits maternal effects is coiling direction of snail shell.The phenotype that is seen in the offspring is controlled by the genotype of the mother.

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If you receive information about an object through your tactile sense, you have?

Answers

Touched it

If you receive information about an object through your tactile sense, you have touched it.

What is tactile sense?Our skin has tactile receptors, which recognize the experience of touch. Our mouth and hands, for example, have more touch receptors than other parts of our skin. Identification of touch, pressure, pain, temperature, and texture depends on the tactile senses.

What senses the sensation of touch?The activation of nerve signals that communicate the body's perception of texture, warmth, and other touch sensations is referred to as tactile stimulation.

What are the four touch senses?Only the pressure sense has its own unique receptors among the thousands of nerve endings in the skin, which respond to the four basic sensations of pressure, hot, cold, and pain. The other four are combined to produce additional feelings.

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Dr. chu studies the environmental influences on gene expression by studying identical twins that have been reared apart. dr. chu's field of study is called:_____.

Answers

Dr. chu studies the environmental influences on gene expression by studying identical twins that have been reared apart. Dr. Chu's field of study is called epigenetics.

Gene expression is the process from which the functional proteins are made from a gene by the processes of transcription and translation. First m-RNA is formed from the DNA and the  it is translated to form the protein. All the proteins formed inside the body are a result of gene expression.

Epigenetics is the study of reversible changes in the gene. These changes are not inherited to the further generations. The environmental factors are the major reason for thee changes. The changes could be methylation, acetylation, etc.

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Which type of molecule never contains a phosphate group? group of answer choices

Answers

Phosphate group is not a part of triglycerides.

What is phosphate?

An anion, salt, functional group, or ester produced from phosphoric acid is referred to as a phosphate in chemistry. Most frequently, it refers to orthophosphate, a product of orthophosphoric acid H3PO4. Its formula is PO₄³⁻.

Triglycerides don't contain phosphate group. They are a type of fats.

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What type of epithelial tissue is most likely to be found lining the respiratory passageway due to its ability to move mucus?

Answers

The pseudostratified columnar epithelium is the most likely to be found lining the respiratory passageway due to its ability to move mucus.

What is a respiratory passageway?

The web of tissues and organs that aids in respiration is termed as the respiratory system.

The majority of the respiratory system is lined with respiratory epithelium, a ciliated pseudostratified columnar epithelium that is absent from the larynx and throat.

Thus, the pseudostratified columnar epithelium is the most likely to be found lining the respiratory passageway due to its ability to move mucus.

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What happens when a virus attacks a cell?

Answers

Answer:

the cell gets infected with the virus I think

Explanation:

it will as the virus will spread to the cell so the cell also turns into a virus

What brain structure enables this water polo player to coordinate his swimming and throwing motions?

Answers

Cerebellum brain structure enables this water polo player to coordinate his swimming and throwing motion.

Cerebellum - A large number of processes and functions in both our brain and body are coordinated and regulated by the cerebellum, which is a component of the brain. Even though it's quite little compared to our entire brain, it contains more than half of the body's neurons. Neurons are the cells that make up your nervous system.

Neurons- The basic building blocks of the brain and nervous system are neurons (neurons or nerve cells). Neurons are the cells that receive sensory information from the outside world, give motor orders to our muscles, and convert and relay electrical impulses at each stage along the way.

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The layer of the embryonic disk that will develop into the muscles, skeleton, circulatory system and other internal organs is called the?

Answers

Any one of the three cellular layers formed during animal embryogenesis and later differentiated to become the various organs and tissues of the organism are referred to as the "germ layer." Three early cell groupings or starting tissues are the ectoderm (cells on the outside), endoderm (cells inside), and mesoderm.

Endoderm is responsible for the development of the lungs, throat, thyroid, parathyroid, stomach, colon, liver, pancreas, urine bladder, the lining of the urethra, epithelial parts of the trachea, and intestines.

The skeletal, connective tissue, urogenital system, heart, blood (lymph cells), spleen, dermis of the skin, crystal lens of the eye, and skeletal muscle are all developed from the mesoderm.

The ectoderm is responsible for the development of the mammary glands, the central nervous system, the eye's lens, the ganglia and nerves, pigment cells, the connective tissues of the head, the epidermis, and the skin.

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Dna helicase inhibitors are well studied as potential drug targets. What would you expect to see if dna helicase activity is inhibited?.

Answers

DNA strands are separated and ATP hydrolysis is catalyzed by the helicase, preventing the unwinding of the double helix.

What is helicase?Nucleic acid or nucleic acid protein complexes can be altered or bound by enzymes referred to as helicases. Helicases are present in both DNA and RNA. DNA helicases are essential for DNA replication because they separate double-stranded DNA into single strands, enabling each strand to be copied. During DNA replication, DNA helicases unwind DNA at regions known as origins, where synthesis will start. The replication fork is a structure that develops as the DNA helicase unwinds the DNA. It is so called because the two strands of DNA appear to fork as they are unzipped apart. The hydrogen bonds that bind the nucleotide base pairs of the double-stranded DNA together must be broken using energy.

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If a total of 2000 kcal were consumed (energy came from 250 grams of carbohydrates, 66 grams of fat, and 102 grams of protein), what percentage of energy comes from carbohydrates, fat, and protein?

Answers

The percentage of energy that comes from carbohydrates, fats and protein are 50%, 30% and 20% respectively.

What are biomolecules and its importance?

Biomolecules are necessary for the functioning of all living things. Many different small and big molecules, including amino acids, vitamins, fatty acids, neurotransmitters, and hormones, make up biomolecules (protein, carbohydrates, nucleic acids, and enzymes). Nucleic acids, lipids, proteins, and carbohydrates make up the four primary categories of biomolecules.

How long can people live without biomolecules?

Lipids, proteins, carbohydrates, and nucleic acids make up the building blocks of life. All life on Earth depends on each and every one of the four groups. A cell or creature could not exist without one of these four molecules.

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In a high-___ work system, a firm's technology, organizational structure, people, and processes work together seamlessly. this results in the firm having an advantage in the competitive environment.

Answers

Answer:performance

Explanation:

Other Questions
Match the word with the correct definition.A. words that create picturesB. a comparisonC. the main ideaD. the attitude of the writer toward the subject, ideas, theme, or charactersE. images, plot patterns, and characters that occur frequently in literatureUse the word bank above to answer the question.11. metaphor (1 point)ABDE 1. hardwoods reproduce using flowers and have broad leaves; hardwoods include trees such as blank, blank, and blank. Variables that are in operation before the display of a behavior and are related to the behavior are:______. Of the big five personality dimensions, the highest correlation with leadership is? If someone pursues a college degree for the love and pleasure of learning, the action is driven by:______. Cody decreased the speed of his car by 3.5 miles per second over a period of 60 seconds Find the average change in speed each second Consumer efficiency theory infers that a business needs to adjust to whatever __________ will increase the shopping efficiency of the average shopper. Communication scholars view the flow of communication to be like a river because _____. Choose the numbers that are equivalent to two and seven fifteenths. fourteen fifteenths, 2.46 repeating where the 6 is repeating, 246.6% fourteen fifteenths, 2.715, 271.5% thirty-seven fifteenths, 2.46 repeating where the 6 is repeating, 246.6 repeating percent where the 6 is repeating thirty-seven fifteenths, 2.715, 271.5% If the total utility from consuming eight units of a product is 123 and the marginal utility of a ninth unit is 3, then the total utility from consuming nine units would be:________ While playing golf james forgets that his sunglasses are on top of his head. as the day goes on, he forgets that he placed them there. this demonstrates the process of? In 1638, galileo published his book two new sciences, which, among other things, helped redefine the science of? is 25 a perfect cube? Last year, 63 new houses were built in a neighborhood. So far this year,Write your answer in simplest form1112of them have been sold. How many new houses have been sold this year?1314 A tennis player serves a ball horizontally at a height h = 2.7 m a distance d = 14.9 m from the net. what minimum speed must the ball be served so as to clear the 1.08 m tall net? Facilities like the ymca and parks and recreation departments are associated with what kind of setting? a. youth setting b. nonprofit setting c. community setting d. athletic setting Becky decorated 144 cakes in 6 days. How many cakes did she decorate in 1 day. What does miller mean when he says that he has "symbolized them all in Hathorne and Danforth"? How to solve math problem 360 miles traveled on 12 gallons of gasoline? (write each rate as a unit rate)